Lactate Dehydrogenase (LDH) Release. Cell culture 3T3 fibroblasts and RAW 264. Operators can conveniently select up to 6 wavelengths at a time for. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). A 0. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Analysis of ALA by Ehrlich’s Reagent Assay. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. Six wells were assayed for each set of conditions and stadard deviations. Molecular Devices SpectraMAX 340 Microplate Reader. 5 mL of 1 M acetate buffer (pH 4. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. The values were corrected for nonspecific background by subtracting the OD 650. Male 8 pin mini Din to Female 9D. View the Molecular Devices website. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. 2 software was used to measure and calculate of the bioactivity activity studies. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. Assay setup for a 384-well low-volume plate. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). FACS analysis. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. Binding affinity for denosumab. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 340 nm / 70 nm. The SPECTRAmax 340PC can measure. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. SpectraMAX 340 microtiter plate reader. To correct for inter. 5. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 312 gain calibration failed. 5. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). The loading equaled the mass of BSA per mass of particles. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. The inhibition percentage was determined by linear regression through ranges of 20 to 30. The ratio of red to green fluorescence intensity was calculated. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. The assays contained 0. Wound healing assay. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). 0. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The reaction mixture was incubated for 5 min. Description 1. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 5. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. Intra- and inter-assay coefficients of variation were lower than 8. 3 mm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. SpectraMax Multi-Mode Microplate Readers. A. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. When necessary, samples were. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. #N207) LAL Reagent Water (Lonza cat. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Rate of fibrinolysis. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. VersaMax reads 96-well and strip-well plates. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. Stat Fax 3200 Microplate Reader. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Data were expressed as the mean percent viable cell vs. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). Spectrophotometer (SpectraMax 340). A. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). 10. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). Spectral Range. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Donor: 620 nm. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Microplate Readers. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. Includes: SpectraMax 340PC384. 4; 100 m M NaCl, 0. Acceptor: 665nm. P. 4. Create custom fluorescence assays without having to purchase expensive filter sets. 6) and 0. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Fully tested with Warranty. The. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. 00 USD. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. Quick View. Crowder, Ziqian Zeng, Alissa N. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. 0 2. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. Example: [Drawer]. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Product Keyword. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. 3 0. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. application or budget. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Immunofluorescence and Aggregation Analysis. Statistical analysis. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. Emission. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 0. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). (Shiga, Japan) and kept for 30 min in the dark. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 2 0. SpectraMax Multi-Mode Microplate Readers. 6 g sodium acetate trihydrate and 2. SpectraMax 190 reads 96-well and strip-well plates. The plate was covered and incubated for one hour at room temperature. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. 6. The VersaMax and SpectraMax 190 read 96-well microplates. 05 ng/mL; progesterone: 2. Although all samples initially were analyzed at the same volume, the volume of sample. Binding of capture antibodies to polystyrene beads and biotinylation. Samples were plated in duplicate (r=0. One plate carrier adapter is provided with the instrument. Each experiment was performed in duplicate. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. 5. reader (SpectraMax 340; Molecular devices, LLc). The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. Data System. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 1. g. 97), and each plate contained at least six positive and six negative controls. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. DNA extraction from blood meal ELISA homogenate. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Bertin Precellys Evolution Touch Homogenizer. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Cytotoxicity was determined by measuring the release of LDH. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The contents of the. 7. Subsequently, the mixture was heated at 100 °C for 10. 10× trypsin 0. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). , Sunnyvale, CA). BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. , Sunnyvale, CA). 306 plate air cal fail,low light. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. Also for: Spectramax 190, Spectramax 340pc384, Versamax. Tunable for filterless flexibility. Call 1-800-745-2710. The first time you replace an OEM lamp you must use the N-34-MLA. , Sunnyvale, CA) at 490 nm. Fibrin clots were formed as described above with some modifications 46. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Both readers feature an 8-channel read head for fast reads and. Manager: Yinghua Chen, PhD Contact Email: yinghua. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. The plate was covered and incubated for one hour at room temperature. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. Microplate Spectrophotometer Operator’s Manual. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. No power cord or accessories included. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. TGFα release was calculated as described in the Results. 2. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). 5. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The light features a slim profile to prevent. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. 5), L-ascorbic acid (25. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Western blot. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. MOLECULAR DEVICE M5. The area under the curve (AUC) for the comparison of the growth rates was determined using. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. TDP-43 stress granular cells were treated with 70% ice-cold. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Nitric Oxide Assay. Wavelength(s) 340 to 850 nm; Add to Compare List. Unit has several areas of damaged plastic. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. 85%. 25–0. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. SpectraMax Multi-Mode Microplate Readers. 2 were considered negative for IgM antibody. Customer Transformation Hub. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. Cell proliferation was measured using the MTT method. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. Details. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 9000-0756. 5% phosphoric acid) in microplates at room temperature for 10 min. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 2. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 2 g/L solution; dilute 1:10 in HBSS. Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. SpectraMax QuickDrop Micro-Volume Spectrophotometer. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Includes: Computer Loaded with Softmax Pro 5. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 3. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The VersaMax and SpectraMax 190 read 96-well microplates. Stat Fax 3200 Microplate Reader. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Molecular Devices Corporation 1311. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. N-34-LB. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Near bottom. 1% N-1-naphtylethylenediamine dihydrochloride, 2. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. 303 unable to cal dark current. 2 Chemical Reagents 1. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Quick View. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 2. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . Molecular Devices SpectraMax 340 PC 384 Microplate Reader. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. The plates were incubated for 4 additional hours at 37°C. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Vessel Sprout Formation Assay. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. Glutathione reductase (GR) activity was measured, according to Smith et al. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Statistical analysis. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. TDP-43 stress granular cells were treated with 70% ice-cold. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. Homeostatic model assessment for insulin. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. Molecular Devices Spectramax 340 Plate Reader Repair. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. 5 g/L-EDTA 0. In this study we used tetanus toxoid (TT) as a well-defined antigen to. 5. The absorbances were being measured at 515 nm. This test was performed in duplicates. Setup recommendations for SpectraMax. Details. SpectraMAX 340. Softmax PRO v5. Anti-proteolytic activity. ). 6) and 0. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. edu Contact Phone: 216-368-8962. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. AoSMCs were seeded into 6-well culture plates. Details. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Bioz Stars score: 86/100, based on 1 PubMed citations. Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. As a common indicator of equine T cell activation, cytokine induction. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Subsequent replacements require only the lamp (N-34-LB) be replaced. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF.